Changes in Microbiota in Type II diabetes

Anita Skovbjerg Hjort-Gregersen s201190
Asiya Mohamad Yusuf Muses 243930
Magnus Harthimmer s233426
Marco Andreis s243116
Rebecca Hjermind Millum s215024

2024-12-03

Humans as a complex ecosystems

Data set history

8 shotgun sequencing metagenomics studies

Merged into a single dataset

Uploaded on Kaggle as CSV

Data selection

Using the whole dataset?

Focusing only on one study?

The WT2D dataset

What is glucose tolerance?

Hallmarks of Diabetes

HDL (“Good Cholesterol”)

  • Lower in Type 2 Diabetes
  • Risk: Heart disease, stroke
  • Factors: Medications, overweight
  • Distribution: Shift to lower values

Hemoglobin A1C

  • Measures: Avg. glucose (10–12 weeks)
  • Threshold: >48 mmol/mol = Type 2 Diabetes
  • Findings: 12.41% of Type 2 Diabetes cases above threshold

BMI

  • Not Significant
  • Waist Circumference: Strong correlation

Hallmark of Type 2 Diabetes

GAD-Antibodies

  • Associated with Type 1 Diabetes
  • Not significant across BMI or glucose tolerance classes

C-Peptide

  • Marker for insulin production
  • Higher in Type 2 Diabetes

Fasting Insulin

  • Higher in Type 2 Diabetes
  • Statistically significant parameter

Statins and diabetes

  • Used as cardiovascular disease treatment
  • Lower cholesterol levels

  • Insulin levels are not affected by statin
  • Lowers colesterol levels
  • Statins not directly linked to type 2 diabetes

Relative abundance data

Are there differences between healthy and diseased?

Are there really differences?

Conclusions